Pig Peptide YY, PYY ELISA Kit from MyBioSource.com

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Pig Peptide YY, PYY ELISA Kit

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Description

Priciple of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PYY. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated PYY. A competitive inhibition reaction is launched between PYY (Standards or samples) and Biotin-conjugated PYY with the pre-coated antibody specific for PYY. The more amount of PYY in samples, the less antibody bound by Biotin-conjugated PYY. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of PYY in the sample. The color development is stopped and the intensity of the color is measured